Sample processing droplet actuator, system and method

摘要

Sample processing droplet actuators, systems and methods are provided. According to one embodiment, a stamping device including a droplet microactuator is provided and includes: (a) a first plate including a path or network of control electrodes for transporting droplets on a surface thereof; (b) a second plate mounted in a substantially parallel orientation with respect to the first plate providing an interior volume between the plates, the second plate including one or more stamping ports for transporting some portion or all of a droplet from the interior volume to an exterior location; (c) a port for introducing fluid into the interior volume between the plates; and (d) a path or network of reference electrodes corresponding to the path or network of control electrodes. Associated systems and methods including the stamping device are also provided.

主权项

1. A method of dispensing at least one sample droplet from a parent droplet, the method comprising:
(a) providing a droplet actuator comprising substrates arranged to form a gap, wherein:
(i) the gap comprises:
(1) the parent droplet comprising a protein in a concentration exceeding 10 mg/mL;(2) an oil filler fluid surrounding the parent droplet, the filler fluid comprising a, surfactant in an amount selected to enhance droplet operations; and(ii) one or more of the substrates comprise electrodes arranged for conducting droplet operations in the gap by electrowetting using the parent droplet; and (b) using the electrodes to conduct the droplet operations comprising dispensing at least one sample droplet from the parent droplet.