Methods and compositions for producing alkanes and alkenes

摘要

Compositions and methods for producing aldehydes, alkanes, and alkenes are described herein. The aldehydes, alkanes, and alkenes can be used in biofuels.

主权项

1. A method of producing an alkane or alkene from an engineered microorganism, the method comprising,
(a) expressing a first nucleotide sequence encoding an acyl-ACP reductase polypeptide that catalyzes the conversion of acyl ACP to a fatty aldehyde in said engineered microorganism, wherein the acyl-ACP reductase polypeptide encoded by the first nucleotide sequence has at least 90% sequence identity to SEQ ID NO: 72; (b) expressing a second nucleotide sequence encoding a decarbonylase polypeptide that catalyzes the conversion of a fatty aldehyde to an alkane or alkene in said engineered microorganism, wherein the decarbonylase polypeptide encoded by the second nucleotide sequence has at least 90% sequence identity to SEQ ID NO:20, each of said first and second nucleotide sequences is operably linked to a promoter; and (c) culturing the engineered microorganism in a culture media containing a carbohydrate carbon source under conditions effective to produce an alkane or alkene in the extracellular environment of the microorganism culture.