Asynchronous magnetic bead rotation sensing systems and methods

摘要

Described herein are various methods, devices and systems for performing asynchronous magnetic bead rotation (AMBR) to detect and monitor cellular growth and/or behavior. Cluster rotation of magnetic particles for AMBR is descried. In particular, described herein are systems for the parallel analysis of multiple wells of a sample plate. Also described herein are methods for controlling the illumination and imaging of rotating magnetic particles.

主权项

1. A method of monitoring cell growth and/or binding by asynchronous magnetic bead rotation (AMBR) in a cluster of magnetic particles, the method comprising:
allowing cells to bind to magnetic particles; forming a cluster of the magnetic particles and bound cells such that the cluster of magnetic particles and bound cells are restrained against individual rotation, but are free to rotate together, wherein forming the cluster comprises forming the cluster by magnetic-field-induced magnetic interaction between the magnetic particles to establish a low interparticle distance between the magnetic particles; applying a rotating magnetic field, wherein the orientation of the rotating magnetic field changes over time and the field intensity is maintained constant, at a driving rate so that the cluster of magnetic particles rotate asynchronously to the applied magnetic field at an asynchronous rotation rate, wherein the rotating magnetic field rotates around an axis and wherein the cluster of magnetic particles and bound cells rotate asynchronously about the axis when the rotating magnetic field is above a critical rotation rate for inducing asynchronous rotation of the cluster of magnetic particles and wherein the critical rotation rate varies depending on the cluster of magnetic particles; and detecting a change in the asynchronous rotation rate indicative of cellular binding and/or growth in the cells.