Bacterial toxin vaccine

摘要

A bacterial toxin protein such as a Shiga toxin protein is efficiently produced using plant cells. The plant cells are transformed using a DNA construct containing DNA encoding a hybrid protein in which the bacterial toxin proteins such as the Shiga toxin proteins are tandemly linked through a peptide having the following characteristics (A) and (B) to produce the bacterial toxin protein in the plant cells: (A) a number of amino acids is 12 to 30; and (B) a content of proline is 20 to 35%.

主权项

1. A hybrid protein comprising:
(a) two or three toxin proteins selected from the group consisting of Shiga toxin proteins, cholera toxin proteins, Escherichia coli heat-labile toxin proteins and combinations thereof, and (b) a peptide having the amino acid sequence represented by SEQ ID NO: 2, 82, or 84 or an amino acid sequence which has at least 90% identity to the amino acid sequence represented by SEQ ID NO: 2, 82, or 84 and which is tandemly linked to the C-terminus of each of the toxin proteins, wherein said hybrid protein causes an immune response when administered to an animal.