Dominant Gene Suppression Transgenes and Methods of Using Same

摘要

Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants, and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are methods for testing allelic variants.

主权项

1. A breeding pair of plants comprising four polynucleotides, wherein
(a) the first plant of said pair comprises (i) a first polynucleotide which encodes a gene product and is operably linked to a promoter A, and (ii) a second polynucleotide which encodes a pIR polynucleotide targeting a promoter sequence B, wherein promoter B is not present in said first plant; and (b) The second plant of said pair comprises (iii) a third polynucleotide which encodes a gene product and is operably linked to said promoter B, and (ii) a fourth polynucleotide which encodes a pIR polynucleotide targeting said promoter A, wherein promoter A is not present in said second plant.