Complexation of Fatty Acid-Conjugated Molecules with Albumin

摘要

The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.

主权项

1. A method of delivering a first molecule to a target within the body of a human, the method comprising
contacting a first composition and a second composition outside the body of the human, wherein the first composition comprises a conjugate of the first molecule and a fatty acid, wherein the second composition comprises a human serum albumin, and wherein the first and second compositions are contacted for a time and under conditions sufficient for the conjugate to bind with the albumin to form a complex; and thereafter administering the complex to a tissue from which the target is accessible, whereby the first molecule is delivered to the target.