发明名称 |
POLYPEPTIDES HAVING IMMUNOACTIVATING ACTIVITY AND METHODS OF PRODUCING THE SAME |
摘要 |
Isolated polypeptides are provided that comprise a cholera toxin B subunit variant having one or more modifications to increase the expression of the polypeptide in a plant cell. Nucleic acids sequences, vectors, and plant cells for expressing the cholera toxin B subunit variant polypeptides are also provided. Further provided are methods for producing the cholera toxin B subunit variant polypeptides that include the steps of transforming a plant cell with a nucleic acid encoding the cholera toxin B subunit variant polypeptides; expressing the variant polypeptides; and purifying the polypeptides. Still further provided are methods of isolating the variant polypeptides that include the steps of obtaining a plant cell expressing the cholera toxin B subunit variant polypeptides; extracting the cholera toxin B subunit variant polypeptides from the plant cell; and purifying the cholera toxin B subunit variant polypeptides. Methods of eliciting an immune response are also provided. |
申请公布号 |
US2014286986(A1) |
申请公布日期 |
2014.09.25 |
申请号 |
US201214005388 |
申请日期 |
2012.03.14 |
申请人 |
Matoba Nobuyuki;Hamorsky Krystal |
发明人 |
Matoba Nobuyuki;Hamorsky Krystal |
分类号 |
C07K14/28 |
主分类号 |
C07K14/28 |
代理机构 |
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代理人 |
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主权项 |
1. An isolated polypeptide, comprising a cholera toxin B subunit variant having one or more modifications to increase the expression of the polypeptide in a plant cell. |
地址 |
Owensboro KY US |