| 摘要 |
<p>Embodiments of the invention provide methods, systems, and devices for detection and measurement of an analyte or analytes. In one embodiment, the invention provides a method for calculating analyte concentrations using a system comprising: at least one assay portion for receiving one or more liquids, the at least one assay portion having a plurality of binding sites over which the one or more liquids can be flowed repeatedly; and a measurement device for measuring binding of one or more analytes in the one or more liquids to the plurality of binding sites, wherein the slope of the binding curves of each binding site, or in the case of non-linear data, the slope at a specified analysis times (the first derivative or tangent), are calculated to provide binding rate data (initial rates) that are then applied to an enzyme catalysis type reaction equation, and compared to the rate-based binding curves for known standards for those analytes previously or concurrently developed.</p> |
