发明名称 |
METHODS FOR AMPLIFICATION OF NUCLEIC ACIDS ON SOLID SUPPORT |
摘要 |
<p>The present invention provides methods for amplifying a nucleic acid from a mixture of nucleic acids utilizing a solid support. Methods are provided utilizing user-defined primer oligonucleotides for directional amplification. Methods are also provided utilizing blocker and displacer oligonucleotides for generating amplified target nucleic acids of defined length. A sample is applied to the solid support and the target nucleic acid within the sample binds a first oligonucleotide affixed to a solid support. A primer sequence containing a target binding region and a polymerase promoter sequence is annealed to the bound target nucleic acid and extended producing a first duplex nucleic acid. The target sequence is removed leaving a first nucleic acid that binds a second oligonucleotide affixed to a solid support. The second oligonucleotide is extended to produce a second duplex nucleic acid that contains a second nucleic acid. The second nucleic acid is amplified by adding a polymerase.</p> |
申请公布号 |
CA2905144(A1) |
申请公布日期 |
2014.09.25 |
申请号 |
CA20142905144 |
申请日期 |
2014.03.14 |
申请人 |
ARNOLD, LYLE J.;NELSON, NORMAN C. |
发明人 |
ARNOLD, LYLE J.;NELSON, NORMAN C. |
分类号 |
C12Q1/68;C12N9/10;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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