VIBRIO CHOLERAE 0139 CONJUGATE VACCINES

摘要

The disclosure pertains to conjugates of the capsular polysaccharide of Vibrio cholerae O139, or a structurally and/or immunologically related oligo- or poly-saccharide, and a carrier. These conjugates are useful as pharmaceutical compositions and/or vaccines to induce serum antibodies which have bactericidal (vibriocidal) activity against V. cholerae, in particular V. cholerae O139, and are useful to prevent, treat and/or reduce the severity of disease caused by V. cholerae infection, such as cholera. The present disclosure also relates to diagnostic tests for V. cholerae infection, and/or cholera caused by V. cholerae infection, using one or more of the oligo- or poly-saccharide-carrier conjugates or antibodies described above.

主权项

1. A method for preparing a conjugate molecule, comprising:
(a) contacting capsular polysaccharide of Vibrio cholerae O139 with adipic acid dihydrazide in the presence of a carboxyl activating reagent; and (b) contacting the product of (a) with a mutated diphtheria toxin in the presence of a carboxyl activating reagent, thereby preparing the conjugate molecule comprising capsular polysaccharide of Vibrio cholerae O139, covalently bound to the mutated diphtheria toxin which functions as a carrier protein, in which the toxin is covalently bound to the polysaccharide by coupling with a dicarboxylic acid dihydrazide linker and wherein the dicarboxylic acid dihydrazide linker comprises adipic acid dihydrazide, whereby, the conjugate elicits serum antibodies vibriocidal to at least Vibrio cholerae O139.