Method and Apparatus for Pyrolysis-induced Cleavage in Peptides and Proteins

摘要

A method and apparatus for conducting the rapid pyrolysis of peptides, proteins, polymers, and biological materials. The method can be carried out at atmospheric pressures and takes only about 5 to 30 seconds. The samples are cleaved at the C-terminus of aspartic acid. The apparatus employs a probe on which the sample is heated and digested components analyzed.

主权项

1. A method of analyzing a peptide sample containing aspartic acid and cysteine, comprising the steps of:
(a) heating the peptide sample to between about 180° C. and about 250° C., in a period of between about 5 seconds and about 30 seconds to cleave the peptide at both the aspartic acid and cysteine site-specific locations; (b) electrospraying the digested sample with a solvent to produce desorbed ions of components of the digested sample; and (c) detecting the desorbed ions.