摘要 |
The invention describes a rapid, simple and effective method for magneto-electrochemical detection of an analyte in a sample without any washing step, comprising the incubation of said sample in presence of magnetic beads and a recognition element linked to an enzyme in which the recognition element and said magnetic particles are capable of interacting independently with said analyte, the application of an electrode to the result of this incubation step in presence of at least one magnet, the addition of an electrochemical substrate for said enzyme to obtain a redox compound, the electrochemical measurement on the electrode surface of said redox compound or at least one product of the redox reaction caused by said compound in presence of the sample components, the added reagents in the incubation and the electrochemical substrate, and the analyte determination evaluating the result of the previous step respect to a reference. |