METHOD FOR OBSERVING LONG-DISTANCE TRANSPORT OF FUSION PROTEIN TO LEAF BUD OF ORYZA SATIVA

摘要

Disclosed is a method for observing long-distance transport of fusion protein to the leaf bud of oryza sativa, the method comprising: using GFP fusion protein to treat the root tip of the oryza sativa, freezing and sectioning the leaf bud of an oryza sativa plant having the root tip treated, dying a leaf bud section by using PI fluorescent dye, and using a confocal microscope to directly observe the transport of the GFP fusion protein from the root tip to the leaf bud of the oryza sativa. The method uses the GFP fusion protein to treat the root tip of the oryza sativa for a length of 0.5 cm, freezes and sections the leaf bud, dyes a leaf bud section, and uses a confocal microscope to observe the transport of the GFP fusion protein from the root tip to the leaf bud of the oryza sativa, thus allowing learning directly and qualitatively about the long-distance transport condition of the GFP fusion protein within the oryza sativa plant, and providing convincing evidence for the ability of effector protein to induce the system defense response of the oryza sativa. In addition, the method is easy, accurate, quick, and of important usage value.