| 发明名称 |
HEAT-RESISTANT NEWCASTLE DISEASE VIRUS LIVE VACCINE VECTOR SYSTEM AND USE THEREOF |
| 摘要 |
A heat-resistant NDV live vaccine vector system includes a transcription plasmid, three helper plasmids, and host cells. The transcription plasmid is constructed by through cloning complete genomic cDNA of a heat-resistant NDV vaccine strain to a pBR322 vector. The three helper plasmids are constructed by cloning sequences coding nucleoprotein (NP), phosphoprotein, large polymerase protein of a heat-resistant NDV vaccine strain respectively to pcDNA3.1 vectors. A recombinant NDV artificially obtained by cotransfacting host cells with the transcription plasmid and the three helper plasmids shows heat-resistance. |
| 申请公布号 |
US2014287496(A1) |
申请公布日期 |
2014.09.25 |
| 申请号 |
US201313924812 |
申请日期 |
2013.06.24 |
| 申请人 |
Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences |
发明人 |
Wen Guoyuan;Shao Huabin;Yang Jun;Wang Honglin;Luo Qingping;Zhang Rongrong;Ai Diyun;Luo Ling;Shang Yu;Guo Jing;Chen Chen |
| 分类号 |
C12N15/86 |
主分类号 |
C12N15/86 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A heat-resistant Newcastle disease virus (NDV) live vaccine vector system, comprising:
a transcription plasmid, comprising a complete genomic cDNA sequence of a heat-resistant NDV strain; three helper plasmids, comprising DNA sequences encoding nucleoprotein (NP), phosphoprotein, and large polymerase protein of the heat-resistant NDV strain; and host cells allowing replication of the heat-resistant NDV strain. |
| 地址 |
Wuhan CN |
