NUCLEIC ACID CONSTRUCT COMPRISING NUCLEIC ACID DERIVED FROM GENOME OF HEPATITIS C VIRUS OF GENOTYPE 3a

摘要

A nucleic acid includes, in the following order, a 5′ untranslated region comprising a particular nucleotide sequence of the genome of hepatitis C virus genotype 3a; a nucleotide sequence encoding a particular amino acid sequence of an NS3 protein, a nucleotide sequence encoding a particular amino acid sequence of an NS4A protein, a nucleotide sequence encoding a particular amino acid sequence of an NS4B protein, a nucleotide sequence encoding a particular amino acid sequence of an NS5A protein, a nucleotide sequence encoding a particular amino acid sequence of an NS5B protein of the hepatitis C virus genotype 3a; and a 3′ untranslated region comprising a particular nucleotide sequence of a genome of hepatitis C virus genotype 3a.

主权项

1. A nucleic acid comprising, in the following order, a 5′ untranslated region comprising a nucleotide sequence of nucleotides 1 to 340 of SEQ ID NO: 1; nucleotide sequences encoding an amino acid sequence of an NS3 protein of amino acids 1033 to 1663, a nucleotide sequence encoding an amino acid sequence of an NS4A protein of amino acids 1664 to 1717, a nucleotide sequence encoding an amino acid sequence of an NS4B protein of amino acids 1718 to 1978, a nucleotide sequence encoding an amino acid sequence of an NS5A protein of amino acids 1979 to 2430, a nucleotide sequence encoding an amino acid sequence of an NS5B protein of amino acids 2431 to 3021 of SEQ ID NO: 14; and a 3′ untranslated region comprising a nucleotide sequence of nucleotides 9407 to 9655 of SEQ ID NO: 1, which are of a genome of a hepatitis C virus of genotype 3a, provided that if the nucleic acid is RNA, thymine (t) in the nucleotide sequence is replaced with uracil (u).