发明名称 |
INCORPORATION OF METHYL LYSINE INTO POLYPEPTIDES |
摘要 |
The invention relates to A method of making a polypeptide comprising at least one Nε-methyl-lysine at a specific site in said polypeptide, said method comprising (a) genetically directing the incorporation of R—Nε-methyl-lysine into said polypeptide, wherein R comprises an auxiliary group; and (b) catalysing the removal of R from the polypeptide of (a). In particular the invention relates to such a method wherein genetically directing the incorporation of R—Nε-methyl-lysine into said polypeptide comprises arranging for the translation of a RNA encoding said polypeptide, wherein said RNA comprises an amber codon, and wherein said translation is carried out in the presence of an amber tRNA charged with R—Nε-methyl-lysine. |
申请公布号 |
US2014287528(A1) |
申请公布日期 |
2014.09.25 |
申请号 |
US201314048684 |
申请日期 |
2013.10.08 |
申请人 |
MEDICAL RESEARCH COUNCIL |
发明人 |
Chin Jason;Nguyen Duy P. |
分类号 |
C07K14/47;G01N33/566 |
主分类号 |
C07K14/47 |
代理机构 |
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代理人 |
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主权项 |
1. A method of making a polypeptide comprising at least one Nε-methyl-lysine at a specific site in said polypeptide, said method comprising
(a) genetically directing the incorporation of R—Nε-methyl-lysine into said polypeptide, wherein R comprises an auxiliary group; and (b) catalysing the removal of R from the polypeptide of (a). |
地址 |
LONDON GB |