发明名称 |
METHOD FOR PRODUCING INSOLUBLE AGGREGATE OF NEURODEGENERATIVE-DISEASE-RELATED PROTEIN |
摘要 |
The purpose of the present invention is to develop a method for producing a large amount of an insoluble aggregate that is equivalent to an insoluble aggregate formed in the brain of a patient. A method of producing an insoluble aggregate of a neurodegenerative-disease-related protein according to the present invention comprises the steps of: (1) introducing an insoluble fraction originated from the brain of a neurodegenerative disease patient into a cultured cell in which the neurodegenerative-disease-related protein can be expressed in a constitutive manner; (2) culturing the cultured cell into which the insoluble fraction has been introduced; and (3) extracting separating an insoluble fraction from the cultured cell. Optionally, the method may additionally comprise a step of amplifying the insoluble aggregate of the neurodegenerative-disease-related protein in the cultured cell. |
申请公布号 |
EP2781601(A1) |
申请公布日期 |
2014.09.24 |
申请号 |
EP20120848991 |
申请日期 |
2012.05.18 |
申请人 |
TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE |
发明人 |
NONAKA, TAKASHI;MASUDA, MASAMI;YAMASHITA, MAKIKO;AKIYAMA, HARUHIKO;HASEGAWA, MASATO |
分类号 |
C12P21/02;C07K14/47;C12N15/09;C12Q1/02;C12Q1/06;G01N33/15;G01N33/50;G01N33/68 |
主分类号 |
C12P21/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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