Cancer tissue-derived cell mass and a process for preparing same

摘要

Disclosed is a novel cell mass derived from a cancer tissue, which can reflect the in vivo behavior of a cancer cell correctly. Also disclosed is a process for preparing the cell mass. Specifically disclosed is a cell mass derived from a cancer tissue, which is an separated product that is isolated from a cancer tissue obtained from an individual as a mass containing at least three cancer cells or a cultured product of the separated product and which can retain a proliferation ability in vitro. The cell mass derived from a cancer tissue is produced by, for example, a preparation process comprising the steps of: treating a pulverized product of a cancer tissue removed from a living body with an enzyme; and selecting and collecting a mass containing at least three cancer cells among from an enzymatic treatment product.

主权项

1. A process for preparing a cancer tissue-derived cell mass comprising the steps of:
treating, with a collagenase-containing enzyme, a pulverized product of a cancer tissue removed from a living body; selecting and collecting a mass containing at least three cancer cells having a diameter or a long diameter of 20 μm to 500 μm or volume average particle size of 20 μm to 500 μm from the enzymatically treated product with a process for assorting the size; and culturing the collected mass to obtain a cultured product that takes an almost spherical or ellipsoidal form.