Methods and compositions related to immunizing against <i>Staphylococcal </i>lung disease and conditions

摘要

Embodiments of the invention include methods and compositions useful in a vaccination strategy capable of neutralizing HIa to provide immunoprotection against S. aureus pneumonia. In certain aspects the invention includes a HIa with reduced toxicity, represented by a recombinant mutant form of HIa (HlaH35L) in which histidine 35 is converted to leucine, which can be used to abrogate the productive assembly of the toxin and protect a subject from staphylococcal pneumonia.

主权项

1. A method of eliciting an immune response in a patient to a staphylococcal bacteria, wherein the patient is hospitalized or will be hospitalized or the patient will undergo surgery and/or be anesthetized, comprising administering to a patient an effective amount of a composition comprising a purified recombinant and attenuated Staphylococcus α-hemolysin (Hla) toxin, wherein the composition contains no more than contaminating amounts of any other Staphylococcus protein.