Nanoparticle sensor for measuring protease activity and method for manufacturing the same

摘要

Disclosed are a nanoparticle sensor for measuring protease activity, for protease imaging, and a method for preparing the same. More specifically, the present invention relates to a nanoparticle sensor for measuring protease activity in which a fluorophore- and a quencher-conjugated peptide substrate is conjugated to a biocompatible polymer nanoparticle. The peptide substrate is specifically lysed by a protease. The sensor according to the present invention is capable of inhibiting emission of fluorescence with high extinctive activity of the quencher on a fluorescent material. But strong fluorescence is specifically emitted only if the peptide substrate is lysed by a specific protease. Therefore, the sensor is especially useful as a method for screening a novel drug such as a protease overexpression inhibitor, and early diagnosis of incurable diseases and various diseases such as autoimmune diseases including cancer, osteoarthritis, rheumatoid arthritis and dementia.

主权项

1. A nanoparticle sensor for measuring protease activity, in which a fluorophore and a quencher are conjugated to a peptide substrate, and the peptide substrate is covalently conjugated to a biocompatible polymer nanoparticle, the peptide substrate beingspecifically lysed by protease,
wherein the protease is selected from a group consisting of matrix metalloproteinases (MMPs); the peptide substrate is selected from a group consisting of Gly-Pro-Leu-Gly-Val-Arg-Gly-Lys-Gly-Gly (SEQ ID NO: 1), Gly-Val-Pro-Leu-Ser-Leu-Thr-Met-Gly-Lys-Gly-Gly (SEQ ID NO: 2) and Gly-Pro-Leu-Gly-Met-Arg-Gly-Leu-Gly-Lys-Gly-Gly (SEQ ID NO: 3); and the biocompatible polymer nanoparticle is a hydrophobic modified glycol chitosan.