Method for detecting compounds containing sulfenic acid using a 1,3-cyclohexanedione-based probe

摘要

The present invention provides compounds of Formula I:;
wherein: R1 is a label (e.g., a detectable group and an anti-tumor agent); L is present or absent and when present is a linking group; and x represents an integer from 1 to 10; or a pharmaceutically acceptable salt thereof. The compounds are useful for, among other things, identifying cysteine sulfenic acids in proteins and monitoring oxidative damage in proteins and cells. Adduct formation can be detected using analytical methods such as electrospray ionization mass spectrometry and fluorescence.

主权项

1. A method of detecting a sulfenic acid containing target compound, comprising:
contacting a compound with a target compound; and then detecting the presence or absence of binding of said compound to said target compound; the presence of binding indicating said target compound is a sulfenic acid containing target compound; wherein said compound is a compound of Formula I:wherein:
R1 is a label selected from the group consisting of biotin, fluorophores, antigens, porphyrins, radioactive isotopes and anti-tumor agents; L is present or absent and when present is a linking group; and x represents an integer from 1 to 10; or a pharmaceutically acceptable salt thereof.