Method for monitoring inhibition of platelet function

摘要

The invention provides a method of monitoring the response of platelets to a cyclooxygenase-1 (COX1) inhibitor such as aspirin. The method involves collecting platelet-containing mammalian blood treated with a COX1 inhibitor; mixing the blood with a COX1-dependent platelet agonist, such as arachidonic acid, monitoring extracellular ATP in the agonist-activated blood to generate a measurement, and comparing the measurement to a standard value. Devices, systems, and kits for carrying out the method are also provided.

主权项

1. A method of monitoring inhibition of platelet function response comprising:
collecting less than 100 μl of platelet-containing mammalian test blood from a patient treated with a platelet inhibitor; mixing the test blood with a platelet agonist to generate agonist-activated test blood; monitoring an extracellular platelet-release-reaction light emission in the agonist-activated test blood to generate a test measurement of the light emission; comparing the measured test light emission to a standard value, wherein the standard value of the light emission is determined by collecting platelet-containing standard blood from the patient; mixing the standard blood with the platelet agonist to generate agonist-activated standard blood; and monitoring an extracellular platelet-release-reaction light emission in the agonist-activated standard blood to generate the standard value, wherein the standard blood is collected from the patient before the patient is treated with the platelet inhibitor; and determining an inhibitory effect of platelet activation based on the comparison of the measured test light emission to the standard value.