Animal product free system and process for purifying a botulinum toxin

摘要

Chromatographic processes and systems for purifying a botulinum toxin from an APF fermentation medium.

主权项

1. A substantially animal protein free (“APF”) process for purifying a botulinum toxin comprising the step of sequentially contacting a plurality of chromatography resins with an aqueous medium containing a botulinum toxin, wherein the plurality of chromatography resins utilized is between two to four chromatography resins, and wherein a first chromatography resin is a cation exchange resin, a second chromatography resin is a hydrophobic interaction resin, and a third chromatography resin is a mixed mode resin, thereby obtaining a purified botulinum toxin.