Compositions and methods for culturing spirochetes

摘要

The present invention relates to methods for culturing spirochetes, in particular Borrelia burgdorferi. The present invention also provides methods of identifying spirochetes present in a biological sample. The present invention further provides methods of diagnosing diseases cause by a spirochete infection, such as Lyme disease, syphilis, and multiple sclerosis. The present invention further provides methods for identifying spirochete susceptibilities to antimicrobials and antimicrobial compositions and cocktails. The present invention also provides methods for treating subjects suspected of having a spirochete infection.

主权项

1. An in vitro method of culturing a spirochete comprising:
(1) inoculating a spirochete culture medium with the spirochete to generate a first culture; (2) incubating the first culture in a first culture vessel in about 3-5% CO2 for about 4-6 days; (3) optionally evaluating the first culture for the presence of spirochetes; and (4) after incubating the first culture in about 3-5% CO2 for about 4-6 days, contacting the CO2 incubated first culture with a matrix-coated support, wherein the spirochete is a species of Borrelia.