Puro-DHFR quadrifunctional marker and its use in protein production

摘要

This invention relates to industrial production of proteins. More specifically, the invention relates to the res-DHFR surrogate marker, which corresponds to a fusion between DHFR and a protein conferring resistance to a toxic compound or conferring a metabolic advantage. The invention further relates to the use of res-DHFR for screening cells for high expression of a protein of interest. The invention is illustrated by the Puro-DHFR surrogate marker, which corresponds to a fusion between the puromycin N-acetyltransferase and dihydrofolate reductase (DHFR).

主权项

1. An isolated Puro-DHFR polypeptide comprising SEQ ID NO: 2, said polypeptide having: (i) puromycin N-acetyl transferase activity; and (ii) dihydrofolate reductase activity.