| 摘要 |
FIELD: medicine.SUBSTANCE: method for estimating the bifidus bacteria and lactic acid bacilli in experimental animal's gastrointestinal tract involves preparing bacterial mutants on dense nutrient media with increasing rifampicin concentrations from 10 mcg·ml. The grown spontaneous mutants are selected, and the dense nutrient rifampicin medium is re-inoculated in the concentration of up to 160 mcg·ml. The mutants characterised by hereditarily stable rifampicin resistance are selected for administration into laboratory animals in the concentration of 150-160 mcg·mlin the nutrient medium. The amount of the administered mutants is calculated. After keeping the animals, the selected faeces are suspended in isotonic sodium chloride. A supernatant is selected, and the dense nutrient medium containing rifampicin 110 mcg·mlis inoculated in a Petri dish. It is incubated for 72 h in the microaerophilic environment at 37°C. The plate count is measured, and the growth-positive bacteria count is re-calculated per 1 g of faeces. A portion of the growth-positive microorganisms of the administered count is determined and used to state the survival.EFFECT: invention enables effectively determining the portion of the positive-growth bifidus bacteria and lactic acid bacilli passed through the animal's gastrointestinal tract.2 dwg, 3 tbl, 3 ex |
