发明名称 |
METHOD FOR METABOLOMIC SAMPLE PREPARATION BASED ON IONIC LIQUID DISPERSIVE LIQUID-LIQUID MICROEXTRACTION |
摘要 |
Provided herein is a method comprising one or more of the following steps: (a) lysing cells of a biological sample and contacting the biological sample with an amount of ionic liquid sufficient to denature intracellular metabolic enzymes in the biological sample to produce a contacted cellular sample; (b) mixing the contacted cellular sample with an organic solvent to produce an ionic liquid-organic solvent composition; (c) mixing the contacted cellular sample with the organic solvent to produce a dispersed microdroplet ionic liquid-organic solvent composition; (d) contacting the ionic liquid-organic solvent composition with an ion exchange composition to produce a second ionic liquid-organic solvent composition; (d) separating the ionic liquid from the organic solvent; and (e) extracting metabolites from the ionic liquid. Kits and systems for practicing the subject methods are also provided. |
申请公布号 |
US2014273080(A1) |
申请公布日期 |
2014.09.18 |
申请号 |
US201414205100 |
申请日期 |
2014.03.11 |
申请人 |
Agilent Technologies, Inc. |
发明人 |
Apffel, JR. James Alexander |
分类号 |
G01N1/30 |
主分类号 |
G01N1/30 |
代理机构 |
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代理人 |
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主权项 |
1. A method comprising:
lysing cells of a biological sample; and contacting the biological sample with an amount of ionic liquid sufficient to denature intracellular metabolic enzymes in the biological sample to produce a contacted cellular sample. |
地址 |
Loveland CO US |