COMPOSITIONS AND METHODS FOR LONG INSERT, PAIRED END LIBRARIES OF NUCLEIC ACIDS IN EMULSION DROPLETS

摘要

The invention provides for methods for uniquely labeling populations of nucleic acids of interest in emulsion droplets using random combinations of oligonucleotides. The labeling methodology of the invention may be used, inter alia, to generate mate pair genomic fragments without the need for circularization. Because the method is independent of circularization, mate pairs can be generated from any length genomic fragment.