| 摘要 |
PURPOSE: A method for preparing a mulberry branch extract is provided to produce a large amount of purified powder containing oxyresveratrol with anti-inflammatory and antiaging functions and to use trans-rasveratrol and moracin as a novel material for functional foods and cosmetic products. CONSTITUTION: A method for preparing a mulberry branch extract containing oxyresveratrol comprises the steps of: steaming mulberry branches for one hour and drying; adding 650 kg of a 75-95% ethanol solution to 100 kg of the dried mulberry branches and extracting by ultrasonic wave at 40-50°C for 12 hours; concentrating the extract by reduced pressure; dissolving the concentrate in a 80% ethanol solution and incubating at room temperature; removing precipitate, adding normal-hexane, and fractioning; removing an upper layer oil and concentrating the lower layer; adding a 20% ethanol solution to the defatted ethanol extract of mulberry branches and suspending; treating the defatted ethanol extract with 10% pectinase and hydrolyzing at 30-40°C for 12 hours to obtain a hydrolysate; fractioning the hydrolysate with an ethyl acetate solvent and concentrating the upper layer by reduced pressure; dissolving the concentrate using the 20% ethanol solution and adsorbing onto diaion HP-20 adsorption ion exchange column; washing the resultant with the 20% ethanol solution; and eluting the resultant with a 40% ethanol solution and concentrating. [Reference numerals] (AA) Dried mulberry branches(100kg); (BB) CNCNF(650kg, 80% ethanol solution, ultrasonic extractor, 50°C, 12 hours, twice); (CC) Filter; (DD) Filtered liquid; (EE, LL) Concentrate by reduced pressure; (FF) Ethanol extract of the mulberry branches(10kg); (GG) Re-dissolve(50kg, ethanol solution); (HH) Incubate day and night; (II) Supernatant; (JJ) Defatting(100kg, normal-hexane); (KK) Lower layer; (MM) Defatted ethanol extract of mulberry branches(8.5kg); (NN) Suspend(50kg, 20% ethanol solution); (OO) Enzyme treatment(4L, pectinex 5 XL, 30~40°C, 12 hours); (PP) Cooling; (QQ) Fraction with a solvent(100kg, ethyl acetate, twice); (RR) Dehydrate(10kg, sodium sulfate anhydrous); (SS) Filter and concentrate by reduced pressure; (TT) Ethyl acetate extract(2.1kg); (UU) Diaion HP-20 column chromatography; (VV) Fraction with 40% ethanol solution; (WW) Fraction with 60% ethanol solution; (XX) Mulberry branch extract I(1.2kg); (YY) Mulberry branch extract II(110g) |
