AFFINITY REAGENTS AND METHODS FOR DETECTION, PURIFICATION, AND PROTEOMIC ANALYSIS OF METHYLATED PROTEINS

摘要

Methyl-lysine affinity reagents created by engineering the 3×MBT methyl-lysine binding domain repeat of lethal (3) malignant brain tumor-like protein 1 (L3MBTL1) are disclosed. In particular, the invention relates to affinity reagents and affinity chromatography media comprising the 3×MBT domain repeat and methods of using such affinity reagents in detection, purification, and proteomic profiling of methylated proteins and peptides.

主权项

1. An affinity reagent that binds to a methylated lysine residue, said affinity reagent comprising one or more engineered 3×MBT domains of one or more L3MBTL1 proteins, wherein each 3×MBT domain consists of residues of a L3MBTL1 protein corresponding to amino acids 190 to 530 of human L3MBTL1 numbered relative to the reference sequence of SEQ ID NO:2.