Detection of Nucleic Acids

摘要

This invention provides compositions, methods, and systems for characterizing, resolving, and quantitating single stranded and double stranded DNA and RNA in-situ. Paired sense and anti-sense probes can signal the presence of double stranded nucleic acids. DNA and RNA can be distinguished in cell and tissue samples by hybridizing with probe sets adapted to highlight differences in these targets in-situ.

主权项

1. A composition comprising:
a first nucleic acid probe comprising a first reporter providing a first signal and comprising a first sequence complementary to a sense strand of a gene; and, a second nucleic acid probe comprising a second reporter providing a second signal and comprising a second sequence complementary to an anti-sense strand of the gene; wherein the first sequence and second sequence are not complementary to each other.