METHODS FOR HIGH THROUGHPUT SPERM SORTING

摘要

This disclosure relates to methods for sorting sperm cells in a microfluidic chip. In particular, various steps are incorporated to align and orienting sperm in flow channels, as well as, to determining sperm orientation and measure relative DNA content for analysis and/or sorting.

主权项

1. A method of sorting sperm comprising the steps of:
flowing sperm through a plurality of flow channels in a microfluidic chip; orienting sperm within the plurality of flow channels; flowing the oriented sperm through an inspection region in the flow channels; interrogating sperm at the at least one inspection region to determine sperm characteristics; differentiating oriented sperm from unoriented sperm in the flow channels; selecting a subpopulation of oriented sperm based on the detected sperm characteristics; and collecting the selected subpopulation of sperm in a collection vessel.