INDUCTION OF AN IMMUNE RESPONSE AGAINST DENGUE VIRUS USING THE PRIME-BOOST APPROACH

摘要

The invention relates to methods for the induction of an immune response to dengue virus. The method of inducing an immune response against dengue virus comprises administration of a non-replicating immunogen followed by a boost with a tetravalent live attenuated viral vaccine. Another aspect of the inventive subject matter is a method of inducing an immune response against dengue virus using a heterologous prime-boost regimen with the priming immunogen comprising a DNA expression system, an adenovirus expression vector or a Venezuelan equine encephalitis virus replicon system and the boosting immunogen comprising the same without the DNA expression system. Each expression system contains DNA sequences encoding dengue viral proteins.

主权项

1. A method of inducing an immune response to dengue virus comprising administering a priming dengue virus immunogen and boosting with a heterologous dengue virus immunogen wherein:
a. said priming dengue virus immunogen is selected from the group consisting of:
i) a DNA expression system;ii) an adenovirus expression vector; andiii) a Venezuelan equine encephalitis virus replicon system; b. said boosting dengue virus immunogen is selected from the group consisting of:
i) an adenovirus expression vector; andii) a Venezuelan equine encephalitis virus replicon system.