CHLAMYDIA-SPECIFIC CD8+ T CELLS AND METHODS OF ISOLATING

摘要

Isolated, Chlamydia-specific, IL-13 expressing CD8+ T cell clones are provided for understanding the biology of Chlamydia infection and screening of therapeutics. Furthermore, methods are provided for isolating CD8+ T cells comprising the steps of infecting or identifying a naturally infected mammal with at least one species of Chlamydia causing bacteria, allowing the bacteria to clear or for a T cell immune response to the natural infection, collecting immune splenocytes from the mammal, providing at least one antigen from a Chlamydia-specific bacteria, and expanding and depleting the CD4+ T cell population or purifying the CD8 T cell population to isolate IL-13 expressing CD8+ T cell clones.

主权项

1. A method for isolating Chlamydia-specific CD8+ T cells comprising the steps of:
recovering immune splenocytes from a mammal previously infected with at least one species of a Chlamydia-causing bacteria; providing the immune splenocytes with at least one antigen to the Chlamydia-causing bacteria; culturing a population of the immune splenocytes under conditions which bring about an increase in the number of CD8+ T cells in the a population and utilizes immune antigen presenting cells; isolating CD8+ T cells from the population; and deriving individual CD8+ T cell clones from the isolated CD8+ T cells, wherein the clones comprise interleukin-13 expressing CD8+ T cell clones.