METHODS FOR MAKING CYTOKINE COMPOSITIONS FROM TISSUES USING NON-CENTRIFUGAL METHODS

摘要

Non-centrifugal methods for generating a solution rich in interleukin-1 receptor antagonist from a tissue comprising cytokine-producing cells. The solution rich in IL-1ra can also include at least one of sTNF-RI, sTNF-RII, IGF-I, EGF, HGF, PDGF-AB, PDGF-BB, VEGF, TGF-β1, and sIL-1 RII.

主权项

1. A method for generating a solution rich in interleukin-1 receptor antagonist (IL-1ra) comprising:
(a) obtaining a cytokine cell suspension by separating cytokine-producing cells from a tissue comprising cytokine-producing cells using a non-centrifugal process; (b) contacting the liquid volume comprising cytokine-producing cells with a solid extraction material; and (c) separating the liquid volume from the solid extraction material to obtain the solution rich in IL-1ra.