| 主权项 |
1. An isolated TIMP-3 mutein having a mature region that is at least 95% identical in amino acid sequence to the mature region of TIMP-3 set forth in SEQ ID NO:2, having at least one mutation, the mutation being selected from the group consisting of:
(a) K45E, K49S; (SEQ ID NO: 5); (b) K45E, K49E; (SEQ ID NO: 6); (c) K45E, T63E; (SEQ ID NO: 7); (d) K45E, Q80E; (SEQ ID NO: 8); (e) K45E, T63E, H78E; (SEQ ID NO: 10); (f) T63E, H78E, Q80E; (SEQ ID NO: 11); (g) K45E, T63E, H78E, Q80E; (SEQ ID NO: 12); (h) T63E, T74E, H78E; (SEQ ID NO: 13); (i) T63E, T74E, H78D; (SEQ ID NO: 14); (j) L51T, T74E, H78D; (SEQ ID NO: 53); (k) T74E, H78E, Q80E; (SEQ ID NO: 16); (l) T74E, H78D, Q80E; (SEQ ID NO: 17); (m) K45N, V47T; (SEQ ID NO: 26); (n) K65N, M67T; (SEQ ID NO: 37); (o) K45N, V47T, T63E, T74E, H78E; (SEQ ID NO: 18); (p) K49N, L51T, T63E, T74E, H78E; (SEQ ID NO: 19); (q) K45E, K49N, L51T, T63E; (SEQ ID NO: 20); (r) K49N, L51T, T74E, H78E; (SEQ ID NO: 21); (s) K49N, L51T; (SEQ ID NO: 27); (t) K50N, V52T; (SEQ ID NO: 30); (u) L51N, K53T; (SEQ ID NO: 54); (v) F57N; (SEQ ID NO: 33); (w) P56N, G58T; (SEQ ID NO: 31); (x) T63N, K65T; (SEQ ID NO: 36); (y) P56N, G58T, T63N, K65T; (SEQ ID NO: 32); (z) K75N, P77T; (SEQ ID NO: 38); (a′) H78N, Q80T; (SEQ ID NO: 39); (b′) K94N, E96T; (SEQ ID NO: 40); (c′) E96N, N98T; (SEQ ID NO: 41); (d′) V97N, K99T; (SEQ ID NO: 42); (e′) D110N, K112T; (SEQ ID NO: 43); (f′) Q126N; (SEQ ID NO: 44); (g′) R138N, H140T; (SEQ ID NO: 46); (h′) R138T; (SEQ ID NO: 45); (i′) T158N, K160T; (SEQ ID NO: 47); (j′) T166N, M168T; (SEQ ID NO: 48); (k′) G173T; (SEQ ID NO: 49); (l′) H181N, A183T; (SEQ ID NO: 50); (m′) R186N, K188T; (SEQ ID NO: 51); (n′) P201N, K203T; (SEQ ID NO: 52); (n′) A208Y; (SEQ ID NO: 55); (o′) A208V; (SEQ ID NO: 56); (p′) K45S, F57N; (SEQ ID NO: 23); (q′) K49S, F57N; (SEQ ID NO: 28); (r′) K68S, F57N; (SEQ ID NO: 34); (s′) K133S, F57N; (SEQ ID NO: 35); (t′) K45S, K133S, F57N; (SEQ ID NO: 24); and (u′) K49S, K68S, F57N (SEQ ID NO: 29). |
