发明名称 |
FRET BASED MULTIPLEX PROBES |
摘要 |
The present invention includes compositions and methods for fluorescence-based multiplex probe to simultaneously detect one or more enzymatic activities comprising: a first enzymatic target having a first end and a second end, wherein the first end of the first enzymatic target is attached to a central body and the second end of the first enzymatic target is attached to a first fluorophore; a second enzymatic target having a first end and a second end, wherein the first end of the second enzymatic target is attached to the central body and the second end of the second enzymatic target is attached to a third fluorophore; wherein the central body comprises at least one second fluorophore; wherein the first enzymatic target comprises a specific cleavage site of a first enzyme that cleaves the first enzymatic target; and wherein the second enzymatic target comprises a specific cleavage site of a second enzyme. |
申请公布号 |
US2014274797(A1) |
申请公布日期 |
2014.09.18 |
申请号 |
US201414206738 |
申请日期 |
2014.03.12 |
申请人 |
Texas Christian University ;University of North Texas Health Science Center at Fort Worth |
发明人 |
Gryczynski Zygmunt;Gryczynski Ignacy;Fudala Rafal;Maliwal Badri P. |
分类号 |
C12Q1/37 |
主分类号 |
C12Q1/37 |
代理机构 |
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代理人 |
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主权项 |
1. A fluorescence-based multiplex probe to simultaneously detect one or more enzymatic activities comprising:
a first enzymatic target having a first end and a second end, wherein the first end of the first enzymatic target is attached to a central body and the second end of the first enzymatic target is attached to a first fluorophore; a second enzymatic target having a first end and a second end, wherein the first end of the second enzymatic target is attached to the central body and the second end of the second enzymatic target is attached to a third fluorophore; wherein the central body comprises at least one second fluorophore; wherein the first enzymatic target comprises a specific cleavage site of a first enzyme that cleaves the first enzymatic target; and wherein the second enzymatic target comprises a specific cleavage site of a second enzyme. |
地址 |
Fort Worth TX US |