TRANSPORTER WITH A GLUCOSE SENSOR FOR DETERMINING VIABILITY OF AN ORGAN OR TISSUE

摘要

An apparatus for perfusing an organ or tissue includes a perfusion circuit for perfusing the organ or tissue with a perfusate; and a sensor operatively connected to the perfusion circuit. The sensor includes a solid support to which is attached a recognition molecule that permits detection of the target agent. The recognition molecule specifically binds to the target agent in the presence of the target agent but not significantly to other agents. The sensor also includes an enzyme that can catalyze the conversion of a substance to glucose. In the presence of the target agent the enzyme can convert the substance into glucose, which can then be detected and optionally measured by the sensor.

主权项

1. An apparatus for perfusing an organ or tissue, the apparatus comprising:
a perfusion circuit for perfusing the organ or tissue with a perfusate; and a sensor operatively connected to the perfusion circuit, which sensor comprises:
a solid support to which is attached a recognition molecule that specifically binds to a viability-indicating target agent that may be in the perfusate;a substance that can be enzymatically converted to glucose;an enzyme that can catalyze conversion of the substance to glucose in the presence of the viability-indicating target agent; anda glucose meter for detecting glucose produced from the substance.