| 主权项 |
1. An isolated polynucleotide encoding a modified beetle luciferase comprising an internal insertion of a heterologous amino acid sequence which interacts with a molecule of interest, wherein the activity of the modified beetle luciferase is altered after the molecule of interest interacts with the insertion sequence relative to the activity before the interaction, wherein the internal insertion is at a residue or region in a beetle luciferase sequence which is tolerant to modification. |
