NUCLEIC ACID AMPLIFICATION REACTION DEVICE AND NUCLEIC ACID AMPLIFICATION METHOD

摘要

A nucleic acid amplification reaction device includes a rotary body having a mounting portion on which a cartridge, which includes a tube that has a plug containing an eluate in which a nucleic acid having bound to nucleic acid-binding solid-phase carriers is eluted from the carriers and a nucleic acid amplification reaction container that is in communication with the tube and contains oil undergoing phase separation from the eluate, is mounted; and a heater forming a temperature gradient inside the nucleic acid amplification reaction container, in which when the posture of the cartridge is changed due to the rotation of the rotary body, a droplet of the eluate introduced from the tube moves inside the nucleic acid amplification reaction container.

主权项

1. A nucleic acid amplification reaction device comprising:
a rotary body having a mounting portion on which a cartridge is mountable, the cartridge including:
a tube that has a plug containing an eluate in which a nucleic acid having bound to nucleic acid-binding solid-phase carriers is eluted from the carriers; anda nucleic acid amplification reaction container that is in communication with the tube and contains oil undergoing phase separation from the eluate; and a heater thermally associated with the nucleic acid amplification reaction container so as to form a temperature gradient inside the nucleic acid amplification reaction container, wherein the rotary body is configured to rotatably change a posture of the cartridge so that a droplet of the eluate introduced from the tube moves inside the nucleic acid amplification container.