METHODS TO ASSESS ENZYME ACTIVITY USING MASS SPECTROMETRIC IMMUNOASSAY

摘要

Methods to assess biological enzyme activity directly in a complex clinical sample. To such a sample, e.g., plasma, an exogenous synthetic peptide substrate for an enzyme present in the sample and to be assessed is added. The sample then undergoes mass spectrometric immunoassay (MSIA) to monitor the mass spectral profile of the immuno-purified exogenous synthetic peptide substrate directly from the sample. Using the resulting profile, parameters such as enzyme activity and effect of enzyme modulators, including therapeutics administered to an individual for a condition involving the enzyme, may be determined.

主权项

1. A method for biological enzyme activity assessment directly in a clinical sample, the method comprising the steps of
(a) adding to a clinical sample in vitro an exogenous synthetic peptide substrate for an enzyme to be assessed in the clinical sample, (b) performing mass spectrometric immunoassay (MSIA) to obtain a mass spectral measurement of a purified exogenous synthetic peptide substrate directly in the sample, and (c) using the mass spectral measurement of the purified exogenous synthetic peptide substrate to obtain at least one parameter indicating the activity of the enzyme in the sample, assessing biological enzyme activity directly in a clinical sample.