METHODS OF CELL CULTURE

摘要

Polypeptide preparations having target levels of glycans, and methods of producing such polypeptide preparations using ammonium and/or lysine, are described.

主权项

1. A method of producing a recombinant protein preparation having a target value of one or more of high mannose glycans, galactosylated glycans, and fucosylated glycans, the method comprising:
(a) providing a cell genetically engineered to express a recombinant protein; (b) culturing the cell in a culture medium comprising ammonium under conditions in which the cell expresses the recombinant protein; and (c) harvesting a preparation of the recombinant protein produced by the cell, wherein the preparation has the target value of one or more of high mannose glycans, galactosylated glycans, and fucosylated glycans.