Reagents, Systems and Methods for Analyzing Erythrocytes and Platelets

摘要

Aspects of the invention include hematology analysis reagents, systems and methods that can be used to preserve blood cell morphology and integrity as well as provide sample integrity and optical clarity to facilitate optical analysis of blood samples. In some embodiments, the reagents include a non-phosphate organic buffer and a sphering surfactant. The pH and osmolality of the reagents may be adjusted to desired ranges. In addition, the reagents can be simply diluted with de-ionized water prior to use.

主权项

1. A system for conducting an optical MCV analysis on a sample of whole blood, the system comprising:
(a) a hematology analyzer, the hematology analyzer comprising:
an excitation source positioned to excite particles within the blood sample;a plurality of detectors including (1) an axial light loss detector positioned to measure axial light loss from the excited blood sample, (2) an intermediate angle scatter detector positioned to measure intermediate angle scatters from the excited blood sample, (3) a polarized side scatter detector positioned to measure large angle polarized side scatters from the excited blood sample, (4) a depolarized side scatter detector positioned to measure large angle depolarized side scatters from the excited blood sample; anda processor configured to:
(I) receive the measurements of (1) axial light loss, (2) intermediate angle scatters, (3) large angle polarized side scatters, (4) large angle depolarized side scatters, and(II) perform an MCV analysis of the blood sample, based on a plurality of data received from the detectors; and (b) a hematology reagent comprising:
a non-phosphate organic buffer;a sphering surfactant; andan osmolality adjustment component, wherein the hematology reagent has sufficient optical clarity to facilitate optical analysis of the sample.