FLOW-THROUGH CELL COUNTING ASSAY

摘要

A method for quantitatively measuring white blood cell (leukocyte) count and/or white blood cell (leukocyte) subsets count involves adding specific antibodies labeled with a marker to the biological fluid sample, capture of white blood cells from a fluid sample by a retainer, removal of other than leukocyte cells and other interfering substances by washing or using specific magnetic beads, and reading the result. The device for use in the present method includes a retainer for leukocyte cells and an absorption pad for taking up all excess washing solution flowing past the sample.

主权项

1. A method for determining of total leukocyte or at least one leukocyte subset in a biological fluid sample, the method comprising the steps of:
(a) adding to said biological fluid sample of an antibody specific to said total leukocyte or to said at least one leukocyte subset, said antibody conjugated with a marker; (b) incubating said biological fluid sample for duration from about 5 min to about 60 min and at a temperature ranging from about 20 degrees Celsius to about 37 degrees Celsius; (c) filtering said biological fluid sample through a retainer filter to bind onto said retainer filter of said leukocytes of said biological fluid sample; (d) washing said retainer filter to remove weakly-bound substances; and (e) reading said marker for determining of said total leukocyte or said at least one leukocyte subset in said biological fluid.