发明名称 |
METHOD FOR MANUFACTURING MULTI-FUNCTIONAL BIO-MATERIAL CONJUGATE USING TWO KINDS OF PARTICLE, AND MULTI-FUNCTIONAL BIO-MATERIAL CONJUGATE MANUFACTURED BY MEANS OF SAME |
摘要 |
Disclosed herein are a method for manufacturing a multi-functional bio-material conjugate used as a biosensor for detecting microorganisms, and the like, and a multi-functional bio-material conjugate manufactured by means of the same. The method for manufacturing a multi-functional bio-material conjugate includes: (a) coating a first nanoparticle having magnetic or fluorescent characteristics with protein; (b) manufacturing a conjugate by adsorbing a second nanoparticle having metallic characteristics onto the first nanoparticle coated with protein; and (c) manufacturing the multi-functional bio-material conjugate by adsorbing a bio-material onto the conjugate. The method for manufacturing a multi-functional bio-material conjugate according to the present invention may prevent precipitation of the nanoparticles, easily immobilize the bio-material, and manufacture a bio-material conjugate having multiple functions, by using two kinds of the particles. In addition, the multi-functional bio-material conjugate manufactured by the present method may be used to detect microorganisms at up to a concentration of 101 cfu. |
申请公布号 |
US2014272945(A1) |
申请公布日期 |
2014.09.18 |
申请号 |
US201214351291 |
申请日期 |
2012.10.12 |
申请人 |
GWANGJU INSTITUTE OF SCIENCE AND TECHNOLOGY ;KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY |
发明人 |
Kim Min-Gon;Sung Yun Ju;Byun Ju Young;Oh Young-Kyoung;Joung Hyou-Arm |
分类号 |
G01N33/543;G01N33/58 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
1. A method for manufacturing a multi-functional bio-material conjugate, the method comprising:
(a) coating a first nanoparticle having magnetic or fluorescent characteristics with protein; (b) manufacturing a conjugate by adsorbing a second nanoparticle having metallic characteristics onto the first nanoparticle coated with protein; and (c) manufacturing the multi-functional bio-material conjugate by adsorbing a bio-material onto the conjugate. |
地址 |
Gwangju KR |