DIGITALLY ENHANCED MICROSCOPY FOR MULTIPLEXED HISTOLOGY

摘要

Disclosed herein are embodiments of imaging biological specimens. An imaging system can include a microscope for directly viewing the biological specimen and a multi-spectral imaging apparatus for outputting digitally enhanced images, near-video rate imaging, and/or videos of the specimen. An imaging system can include a digital scanner that digitally processes images to produce a composite image with enhanced color contrast of features of interest.

主权项

1. An multispectral imaging system for imaging a specimen located on a microscope slide, the multispectral imaging system comprising:
an imaging apparatus including
an illuminator including a plurality of different color light sources that sequentially produce light for sequentially illuminating at least a portion of the specimen,an image capture device positioned to capture a plurality of specimen images each corresponding to the specimen being exposed to light from a respective one of the light sources, anda processing device configured to produce contrast enhanced color image data and/or spectrally deconvolved image data based on the specimen images captured by the image capture device; at least one lens positioned along an optical path extending from the specimen to the image capture device; and a display in communication with the imaging apparatus and configured to display contrast enhanced output and/or spectrally deconvolved output of the specimen based on the contrast enhanced color image data and/or spectrally deconvolved image data from the processing device.