Methods and compositions for selective labeling of different biotinylated targets within multicolor or multilabel assays

摘要

Disclosed are compositions and methods for the labeling of two or more targets with different labels. Specifically, disclosed are compositions for biotin and the protection of biotin within multilabel assays which employ the biotin-biotin binding protein binding relationship for each distinct label in relation to targets such as nucleic acids, polypeptides, antibodies or cells. These multilabel assays are enabled through the use of biotin with desthiobiotin, orthogonal protecting schemes for biotin, or a combination of the approaches.

主权项

1. A method of labeling a plurality of targets with a plurality of labels, the method comprising:
providing a plurality of targets, wherein the plurality of targets comprises a first target and a second target, wherein the first target includes a first biotin molecule, and wherein the second target includes a second biotin molecule that is protected by a protecting group; binding the first biotin molecule with a first biotin binding protein, wherein the first biotin binding protein includes a first label; deprotecting the second biotin molecule, and binding the deprotected second biotin molecule with a second biotin binding protein, wherein the second biotin binding protein includes a second label, and wherein the first label is distinguishable from the second label.