METHOD OF CRYOPRESERVING CELLS

摘要

A non-linear cryopreservation protocol and method for improving cryopreservation protocols for cells involves producing a simulation of cellular responses to a range of cooling parameters; determining optimal cooling parameters required to minimize cryoinjury to the cells; and incorporating optimal parameters into the protocol. The simulation is based on mathematical models of cellular parameters. A non-linear cryopreservation protocol for cryopreserving stem cells is also diclosed.