Promoters exhibiting endothelial cell specificity and methods of using same for regulation of angiogenesis

摘要

Isolated polynucleotide sequences exhibiting endothelial cell specific promoter activity, novel cis regulatory elements and methods of use thereof enabling treatment of diseases characterized by aberrant neovascularization or cell growth are disclosed.

主权项

1. A method of inhibiting angiogenesis in an endothelial tissue of a subject in need thereof, the method comprising administering directly to the endothelial tissue or systemically to said subject a vector comprising:
(a) a promoter functional in the endothelial tissue, which comprises a cis-acting regulatory element comprising the sequence as set forth in SEQ ID NOs: 15 and 16, SEQ ID NO: 15 being upstream of SEQ ID NO: 16 in the promoter, or the complementary sequences thereof; and (b) a nucleic acid sequence encoding an apoptosis inducing factor under control of said promoter,wherein said subject is being treated with radiation therapy, chemotherapy, or a combination thereof; and wherein said apoptosis inducing factor is expressed in or near the endothelial tissue and the expression of said apoptosis inducing factor downregulates angiogenesis in the endothelial tissue.