Embryonic-like stem cells derived from adult human peripheral blood and methods of use

摘要

The present invention is related generally to embryonic-like stem cells isolated from adult human peripheral blood, designated herein as peripheral blood-stem cells (PB-SC), which display the characteristics of embryonic stem cells and hematopoietic cells. These cells have the capability of proliferation and are able to differentiate to other types of cells. These cells are, therefore, suitable for use in stem cell-based therapies, particularly autologous stem cell therapies, for the treatment of various diseases such as neurodegenerative diseases, autoimmune diseases, diabetes, spinal cord damage, multiple sclerosis, cardiovascular disease, stroke and birth defects.

主权项

1. A method of harvesting embryonic-like stem cells from peripheral blood comprising:
extracting peripheral blood comprising peripheral blood mononuclear cells (PBMCs); culturing the PBMCs in growth medium within a vessel having at least one hydrophobic surface, such that the PBMCs revert to embryonic-like stem cells and adhere to the hydrophobic surface; obtaining an embryonic-like stem cell population from the PBMCs that are attached to the hydrophobic surface; and isolating embryonic-like stem cells having characteristics comprising positive for CD45 marker and at least one of Oct-4, Nanog, Sox-2, and CD117 marker, negative for CD14 and CD34 markers and at least one of CD3, CD20, CD11c, and CD 11b/Mac-1 marker.